Chuong Lab – Sample Movies
The time-lapse videos are proof-of-principle recordings for
avian skin system. We are still improving the imaging conditions.
dermal condensation of a developing feather bud. Fluorescent images taken from E7 chick skin
explant with dermal cells labeled with H2BGFP through RCAS. Recording time:
14.5 hours. Playback time: 24 sec. Time compression rate: 2175 X.
This one is from supplement movie 7 in Plikus et al., 2011. Self-organizing and stochastic
behaviors during the regeneration of hair stem cells. Science 332:586-589. It illustrates a predictive large-scale modeling showing multiple initiation
sites of hair follicle activation. Two types of plots are shown: the plot on
the right shows each automaton state as a different color (P – blue, A – yellow, R – red, C – green) while the plot
on the left shows expected pigmentation states as they would appear on the skin
(P – grey, A – black, R and C – white). This
model is based on Cellular Automata. In this case each automaton represents one
hair follicle. For this proposal, we will make each automaton represent a
single cell. Coupling of hair follicles will become adhesion between cells. We
will set a threshold so some of those succeed to reach a certain size will be
designated as a successful dermal condensation and should not continue to merge
(as seen in hair wave). We certainly need to modify and develop a new
model with new algorithms, but the general idea is like this.
Melanocyte cell migration in feather buds. Note that
melanocytes are moving toward the distal end of the bud and become blacker
during the process. Image taken from E8 quail dorsal skin.
Recording time: 10 hrs. Playback time: 24 secs. Time
compression rate: 1500X.
Melanocyte cell migration in feather filaments. Note that melanocyte move toward distal and anterior side of the
feather filament, but avoid marginal plate region. Image taken from E8 quail dorsal skin. Recording time: 4 hrs. Playback time: 10 secs.
Time compression rate: 1440 X.