General techniques

Cell culture, Molecular cloning, Cell line construction, Lentivector production and transduction, Recombinant monoclonal antibody production and purification, Multicolor flowcytometry analysis, T and B lymphocytes purification, BMDC preparation, Mouse immunization and analysis, In vivo tumor study, mRNA display, FPLC, HPLC.

Analytic assays

RT-qPCR, Elisa, ELISpot, Cell proliferation assay, Multicolor surface marker and intracellular staining, SDS-PAGE, Western Blot.

Animal procedures

Dissection (Spleen, Lymphnode, Bone marrow, Gut), Eye bleeding, Injections (IV, IP, SC, Oral Gavage), Subcutaneous implantation, Perfusion.

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